IMAGE: Based on ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS) scientists at the Technical University of Munich (TUM) and the Leibniz-Institute for Food Systems Biology have developed a new methodology for… view more
Credit: Andreas Dunkel / Leibniz-Institute for Food Systems Biology
Scientists at the Technical University of Munich (TUM) and the Leibniz-Institute for Food Systems Biology have developed a new methodology for the simultaneous analysis of odorants and tastants. It could simplify and accelerate the quality control of food in the future.
Whether a food tastes good or not is essentially determined by the interaction of odors and tastants. A few trillionths of a gram per kilogram of food is enough to perceive some odorants. Tastants, on the other hand, we only recognize at significantly higher concentrations.
In order to guarantee consistent sensory quality, it is very important for manufacturers to know and control the characteristic odor and taste profiles of their products from the raw material to the finished product. This requires a fast but precise food analysis.
Tastants and aroma substances, however, differ greatly in their chemical and physical properties. As a result, food chemists currently use very different methods to determine the exact nature and quantity of odorants and tastants in a raw material or food. Especially aroma analyses are very time-consuming and therefore expensive. This limits the high-throughput analysis of numerous samples.
One methodical approach for two different substance classes
Thomas Hofmann, Director of the Leibniz-Institute for Food Systems Biology and Professor of Food Chemistry and Molecular Sensory Science at the TUM, explains: “We have now developed a new, innovative methodical approach that will enable us to examine food simultaneously for both odorants and tastants in a time-saving high-throughput process. It is based on an ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS) method typically used for taste analysis.
The new and time-saving feature of the developed approach is that volatile odorous substances can now also be analyzed by means of an upstream enrichment or substance conversion step using this method, which is otherwise not used for aromatic substances.
Apple juice as a test object
“We have tested our new methodological approach using apple juice as an example. The results are very promising,” says Andreas Dunkel, Senior Scientist at the Leibniz-Institute of Food Systems Biology. Together with doctoral student Christoph Hofstetter from the TUM, he was substantially involved in the development of the new approach.
According to the scientists, the new method makes it possible for the first time to analyze a large number of samples in a very short time with regard to their taste and odor giving ingredients.
Also suitable for food profiling
The researchers hope to be able to further develop the method so that it can be used by food manufacturers in the future to quickly and easily monitor the flavor of food along the entire value chain and, if necessary, optimize it.
Last but not least, the new method could also be used to stop food fraud. “Using the identified flavor profiles, it would be possible to check the origin and quality label of the manufacturers and detect food fraud,” says food profiler and food chemist Andreas Dunkel.
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The scientific project was carried out within the framework of the “enable” competence cluster of nutrition research (https:/
Publication:
Christoph Konrad Hofstetter, Andreas Dunkel and Thomas Hofmann:
Unified Flavor Quantitation: Toward High-Throughput Analysis of Key Food Odorants and Tastants by Means of Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry
J. Agric. Food Chem., July 9, 2019 – DOI: 10,1021/acs.jafc.9b03466